Introduction to Immunohistochemistry and in-situ Hybridization

1 NSH Contact Hour(s)

(based on 2 customer ratings)

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Traci DeGeer, BS, HT(ASCP)HTL,QIHC, Ventana Medical Systems, A Part of the Roche Group, Tucson, AZ

Prepared tissue sections are colorless and they are difficult to use to identify cell types and interpret morphologic changes. For this reason, colored dyes such as hematoxylin and eosin are routinely used to identify tissue structures such as nuclear detail, some cells types, smooth and skeletal muscle and connective tissue. The H&E stain, however, does not provide information with regard to specific protein expression. Immunohistochemistry (IHC) is a method that uses antibodies that are generated in animals against proteins of interest. Detection chemistry and chromogens that are optimized in the IHC protocol are used to label the site of antibody binding. This information is used to understand drug target expression in research studies or for clinical diagnostics to improve patient care. In-situ hybridization (ISH) is a method that labels RNA or DNA sequences with complimentary probe sequence. These data are used to understand the relationship of protein and gene expression in the context of tissue sections. Microscopic evaluation and image analysis of IHC and ISH results are used to obtain semi-quantitative data that provides a better understanding of tissue biology. Attendees will gain an understanding of the basic steps of IHC and ISH protocols and their application to research and diagnostic studies.

See more courses in: Immunohistochemistry; Molecular; Clinical; Veterinary/Research

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Continuing Education Credits

NSH Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number VIR111215

Objectives

  • Discuss how antibodies are used in the drug discovery process
  • Illustrate some of the challenges of using immunohistochemistry in the drug discovery process
  • Describe how in situ hybridization is used in the drug discovery process
  • List some of the challenges of using in situ hybridization in the drug discovery process
  • Compare the use of each method in the drug discovery process

Customer Ratings

(based on 2 customer ratings)

Additional Information

Course Description: Prepared tissue sections are colorless and they are difficult to use to identify cell types and interpret morphologic changes. For this reason, colored dyes such as hematoxylin and eosin are routinely used to identify tissue structures such as nuclear detail, some cells types, smooth and skeletal muscle and connective tissue. The H&E stain, however, does not provide information with regard to specific protein expression. Immunohistochemistry (IHC) is a method that uses antibodies that are generated in animals against proteins of interest. Detection chemistry and chromogens that are optimized in the IHC protocol are used to label the site of antibody binding. This information is used to understand drug target expression in research studies or for clinical diagnostics to improve patient care. In-situ hybridization (ISH) is a method that labels RNA or DNA sequences with complimentary probe sequence. These data are used to understand the relationship of protein and gene expression in the context of tissue sections. Microscopic evaluation and image analysis of IHC and ISH results are used to obtain semi-quantitative data that provides a better understanding of tissue biology. Attendees will gain an understanding of the basic steps of IHC and ISH protocols and their application to research and diagnostic studies.
Additional Information: This webinar was presented in November 2015 as part of the NSH Veterinary & Research Scientist Webinar Series.

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