High Complexity Multiplexing

1 NSH Contact Hour(s)

(based on 1 customer ratings)

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Traci DeGeer, BS, HT(ASCP)HTL,QIHC, Ventana Medical Systems, A Part of the Roche Group, Tucson, AZ created for the NSH Laboratory Webinar Series

Many laboratories today have combined two antibodies together in the same tissue in order to assist the pathologist with diagnosis or for use in research. This lecture will discuss the principles of moving beyond combining two antibodies and two colors. Some methodologies for multiplexing are quite straight forward and others require more thought and planning. Both methodologies will be covered during this presentation.

See more courses in: Immunohistochemistry; Clinical; Veterinary/Research

Continuing Education Credits

NSH Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number WEB1116

Objectives

  • Understand why multiplexing is important
  • Explain key components of running a multiplex immunohistochemistry stain
  • Summarize the importance of running proper drop-out controls during denaturation

Customer Ratings

(based on 1 customer ratings)

Additional Information

Course Description:  Many laboratories today have combined two antibodies together in the same tissue in order to assist the pathologist with diagnosis or for use in research. This lecture will discuss the principles of moving beyond combining two antibodies and two colors. Some methodologies for multiplexing are quite straight forward and others require more thought and planning. Both methodologies will be covered during this presentation.
Presenter Bio: Traci DeGeer is Discovery Training Manager for Roche Molecular Systems where she develops training programs for researchers and Roche training sites worldwide. Traci received her bachelors of science in Biology from Radford University in Virginia. She is certified both as a HT and a HTL and has her QIHC. At the start of her career she worked in the clinical setting as a Histotechnologist, Lead Histotechnologist. She then moved to Ventana Medical Systems in 2004 as a technical customer care executive and 8 months later into training. Traci has performed numerous workshops at both the state and national level on a variety of different topics. Traci is an active member of the NSH where she serves as chair of the education and CEU committees. 
Additional Information:  This webinar was presented in November 2016 as part of the annual NSH Laboratory Webinar Series.

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