Muscle Biopsy Techniques

1 NSH Contact Hour(s)
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Konnie Zeitner, HT(ASCP) HTL, SLS’ Safety Consultant, Bellevue, NE created for the NSH Laboratory Webinar Series

Muscle biopsies can present a challenge to the routine histology laboratory. Preparation of muscle biopsies can be a challenge to gross dissection and histology lab personnel as the initial handling is critical for producing optimal results. In this webinar I will discuss some techniques for gross dissection and preparation, freezing, and fixation of the muscle biopsy specimen and will include frozen section cutting and trouble shooting. I will end with a discussion of common stain panels used for clinical diagnosis.

See more courses in: General Histology; Clinical

Continuing Education Credits

NSH Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number WEB0416

Objectives

  • Specimen collection
  • Initial handling in gross dissection
  • Preparation and method for flash freezing
  • Fixation for paraffin and EM processing
  • Frozen sections
  • Trouble shooting

Additional Information

Course Description:  Muscle biopsies can present a challenge to the routine histology laboratory. Preparation of muscle biopsies can be a challenge to gross dissection and histology lab personnel as the initial handling is critical for producing optimal results. In this webinar I will discuss some techniques for gross dissection and preparation, freezing, and fixation of the muscle biopsy specimen and will include frozen section cutting and trouble shooting. I will end with a discussion of common stain panels used for clinical diagnosis.
Presenter Bio:  Konnie Zeitner was employed at the Nebraska Medical Center in Omaha, NE 44 years in the Anatomic Pathology laboratory. There she worked in routine Histology, Immunohistochemistry, Neuropathology and was the Anatomic Pathology manager. She ended her career in the Neuropathology/Muscle biopsy lab where I also handled all the CJD/prion cases before becoming a consultant.
Additional Information: This webinar was presented in April 2016 as part of the annual NSH Laboratory Webinar Series.

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