Developing Immunohistochemical (IHC) Assays for Animal Models of Disease

1 NSH Contact Hour(s)
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Presented by Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC, Premier Laboratory, LLC

In a wide variety of research settings we are tasked to develop immunohistochemical (IHC) assays that will be specifically used for animal models. This webinar will discuss the detailed methods necessary to successfully develop and verify these assays for both well characterized and novel targets.

See more courses in: Immunohistochemistry

Continuing Education Credits

NSH Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number VIR121517
Florida Board of Clinical Laboratory Personnel Credit Hours - Immunohistochemistry/Advanced Histologic Techniques: 1 hour(s)
Course number 20-630855, approved through 1/1/2019

Objectives

  • The importance of creating a collaborative environment between the researcher/pathologist and the histology laboratory.
  • The value of understanding the animal model and the type of data that is to be generated from the histology process.
  • How to research and select the right antibody and control tissues.
  • The use of pilot samples for protocol optimization.
  • How to develop standardized study specific methods for proper tissue collection, fixation, processing, sectioning and IHC staining for ongoing projects.

Additional Information

Course Description: In a wide variety of research settings we are tasked to develop immunohistochemical (IHC) assays that will be specifically used for animal models. This webinar will discuss the detailed methods necessary to successfully develop and verify these assays for both well characterized and novel targets. Topics discussed will include;  1. The importance of creating a collaborative environment between the researcher/pathologist and the histology laboratory, 2. The value of understanding the animal model and the type of data that is to be generated from the histology process, 3. How to research and select the right antibody and control tissues, 4. The use of pilot samples for protocol optimization, 5. How to develop standardized study specific methods for proper tissue collection, fixation, processing, sectioning and IHC staining.
Presenter Bio:  Elizabeth Chlipala is an owning partner, at Premier Laboratory, LLC, a contract histology laboratory in Longmont, Colorado. She has over thirty years’ experience working in laboratories and hospitals, doing both clinical and research histology and immunohistochemistry. She is a member of NSH, ASCP, CSH, Colorado Bioscience Association, and Digital Pathology Association.
Addtional Information: This course was presented in December 2017 as part of the VIR Webinar Series.

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