Multiplexing on Animal and Xenograft Tissue, All Things Considered

1 NSH Contact Hour(s)
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Presented by Andrea Transou, HT(ASCP), Henry Ford Health System

Every doctor and investigator desires to know three things: can the task required be completed, how long will it take, and how much will it cost? Multiplexing is an immunohistochemical technique that allows for the visualization of multiple antigens on the same slide. The ability to visualize multiple antigens through fluorescence or chromogenic staining saves time, money, tissue, and reagents. In order to do this successfully, it is necessary to know how to properly develop a protocol. Issues such as background staining, non-specific staining, species to species reactivity, and a lack of protocol development tactics can hinder the efficient use of multiplexing. This webinar will walk histology professionals through antibody and reagent selection for all tissue types with consideration of species to species reactivity. It will also address concerns of proper control material selection, antigen retrieval, chromogen usage, and stain order. Developing a successful multiplexing protocol can be a daunting task, but with the tools from this webinar anyone can accomplish it.

See more courses in: Immunohistochemistry

Continuing Education Credits

NSH Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number VIR121317
Florida Board of Clinical Laboratory Personnel Credit Hours - Immunohistochemistry/Advanced Histologic Techniques: 1 hour(s)
Course number 20-630845, approved through 1/1/2019

Objectives

  • Read and understand antibody datasheets and build protocols based on the information provided.
  • Learn steps and reagents available to limit species to species reactivity when using an antibody from the same species being stained
  • Understand what factors to consider when building a protocol for multiplexing and how to apply that understanding to troubleshoot prospective pitfalls.

Additional Information

Course Description: 

Every doctor and investigator desires to know three things: can the task required be completed, how long will it take, and how much will it cost? Multiplexing is an immunohistochemical technique that allows for the visualization of multiple antigens on the same slide. The ability to visualize multiple antigens through fluorescence or chromogenic staining saves time, money, tissue, and reagents. In order to do this successfully, it is necessary to know how to properly develop a protocol. Issues such as background staining, non-specific staining, species to species reactivity, and a lack of protocol development tactics can hinder the efficient use of multiplexing. This webinar will walk histology professionals through antibody and reagent selection for all tissue types with consideration of species to species reactivity. It will also address concerns of proper control material selection, antigen retrieval, chromogen usage, and stain order. Developing a successful multiplexing protocol can be a daunting task, but with the tools from this webinar anyone can accomplish it.
Presenter Bio:  Andrea Transou is the leader of the Hermelin Brain Tumor Center at Henry Ford. She holds her HT and QIHC and a BS in Biological Sciences, and has been published in multiple journals including JOVE, the Journal of Neurosurgery, Trends in Cancer Research and Neoplasia.
Additional Information: This course was first presented in Decebmer 2017 as part of the VIR Webinar Series. 

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