Additional Information
Course Description: Large, 3-dimensional datasets are commonplace now with platforms like selective plane illumination (SPIM), spinning disk microscopy, and serial section imaging. Reconstructing 3-dimensional visualizations from these datasets can be cpu and memory intensive, but can be done with relatively simple tools that are freely available. Here we will look at reconstructing a sectioned dataset using FiJi. We will also take a look at finding and quantifying cells automatically on a 3-dimensional confocal dataset.
Presenter Bio: Dr. McKinney received his PHD in physics from the University of Illinois and Sean is a microscopy Group Head at the Stowers Institute for Medical Research, where he manages all daily operations as well as working on research projects. He has built the Institute’s homemade SPIM and PALM rigs and has developed fluorescent proteins.
Additional Information: This was presented as part of the 2017 VIR webinar series.