An Automated Approach to Multiplex, Fluorescent, In Situ Hybridization for mRNA Detection

1 NSH Contact Hour(s)

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Presented by David Krull, HT(ASCP)QIHC, GlaxoSmithKline

A new generation of approaches to in situ hybridization (ISH) using vendor kits and automated platforms has emerged over recent years. What started out as manual, single color, chromogen methods, has expanded to include dual chromogenic and fluorescent reporters. As refinements were made these time consuming, manual methods, were automated allowing for greater throughput and accessibility. The most recent technologic advancement allows the user to fully automate a 4-color fluorescent ISH assay. Since this work can be done in a single tissue section, it enables a greater understanding of mRNA relationships within the tissue microenvironment. This webinar will present our experience in running the assay for the first time and the challenges in developing a validated assay for clinical research.

See more courses in: Molecular

Continuing Education Credits

NSH Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number IHC0717

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(based on 1 customer ratings)

Additional Information

Course Description: A new generation of approaches to in situ hybridization (ISH) using vendor kits and automated platforms has emerged over recent years. What started out as manual, single color, chromogen methods, has expanded to include dual chromogenic and fluorescent reporters. As refinements were made these time consuming, manual methods, were automated allowing for greater throughput and accessibility. The most recent technologic advancement allows the user to fully automate a 4-color fluorescent ISH assay. Since this work can be done in a single tissue section, it enables a greater understanding of mRNA relationships within the tissue microenvironment. This webinar will present our experience in running the assay for the first time and the challenges in developing a validated assay for clinical research.
Presenter Bio: David Krull is team leader in the Exploratory Biomarker Group at GSK. He has 27 years of experience in drug development with special focus on histopathology endpoints. David is the current NSH VIR Chair and is an active member of the IHC and S/C Program Committees. In his free time he enjoys gardening and his newest hobby beekeeping.
Additional Information: This webinar was presented in July 2017 as part of the annual NSH IHC & Molecular Webinar Series.

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